Delayed Signs of UV-C Damage to Chlorella sp. Observed through Fluorescent Staining

Ultraviolet (UV-C) irradiation is the most important part of water filtration, which has no side effects on environment and been used in purification systems aquaculture transistor industries. In this research, effect UV-C Chlorella sp. was investigated. irradiated 0, 1, 2 or 3 times at a fixed flow rate 6.5 L min−1 LED apoptosis death were analyzed by cytometry after staining cells with nucleic acid dye SYTOX Green membrane-associated protein stain Annexin V-PE Reagent. As result irradiation, underwent phosphatidylserine (PS) ectropion plasma membrane damage, resulted death. The proportional to number irradiation. Three doses 91.76 ± 3.33% rate, as observed through staining, rebound within 72 h. This research first report observe that delayed cellular occurred sp., we expect our study can be standard reference for future industrial applications.